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Surface Topography of Polyethylene Glycol Shell Nanoparticles Formed from Bottlebrush Block Copolymers Controls Interactions with Proteins and Cells

Though poly(ethylene glycol) (PEG) is usually utilized in nanoparticle design, the influence of floor topography on nanoparticle efficiency in biomedical purposes has obtained little consideration, regardless of exhibiting vital promise within the examine of inorganic nanoparticles.
Management of the floor topography of polymeric nanoparticles is a formidable problem because of the restricted conformational management of linear polymers that kind the nanoparticle floor. On this work, we set up a simple technique to exactly tailor the floor topography of PEGylated polymeric nanoparticles based mostly on tuning the structure of shape-persistent amphiphilic bottlebrush block copolymer (BBCP) constructing blocks.
We reveal that nanoparticle formation and floor topography may be managed by systematically altering the structural parameters of BBCP structure. Moreover, we reveal that the floor topography of PEGylated nanoparticles considerably impacts their efficiency.
Particularly, the adsorption of a mannequin protein and the uptake into HeLa cells have been carefully correlated to floor roughness and BBCP terminal PEG block brush width. General, our work elucidates the significance of floor topography in nanoparticle analysis in addition to gives an strategy to enhance the efficiency of PEGylated nanoparticles.

Combining Fast Microfluidic Mixing and Three-Colour Single-Molecule FRET for Probing the Kinetics of Protein Conformational Modifications

Single-molecule Förster resonance power switch (FRET) is nicely fitted to finding out the kinetics of protein conformational adjustments, owing to its excessive sensitivity and talent to resolve particular person subpopulations in heterogeneous techniques. Nonetheless, the most typical strategy using two fluorophores can solely monitor one distance at a time, and using three fluorophores for concurrently monitoring a number of distances has largely been restricted to equilibrium fluctuations. Right here we present that three-color single-molecule FRET may be mixed with speedy microfluidic mixing to analyze conformational adjustments in a protein from milliseconds to minutes. Together with handbook mixing, we prolonged the kinetics to 1 h, similar to a complete vary of 5 orders of magnitude in time.

We studied the monomer-to-protomer conversion of the pore-forming toxin cytolysin A (ClyA), one of many largest protein conformational transitions recognized. Web site-specific labeling of ClyA with three fluorophores enabled us to observe the kinetics of three intramolecular distances on the identical time and revealed a beforehand undetected intermediate. The mix of three-color single-molecule FRET with speedy microfluidic mixing thus gives an strategy for probing the mechanisms of complicated biomolecular processes with excessive time decision.

 

Most cancers immunotherapy by NC410, a LAIR-2 Fc protein blocking human LAIR-collagen interplay

 

Collagens are a main element of the extracellular matrix and are useful ligands for the inhibitory immune receptor leukocyte related immunoglobulin-like receptor (LAIR)-1. LAIR-2 is a secreted protein that may act as a decoy receptor by binding collagen with larger affinity than LAIR-1. We suggest that collagens promote immune evasion by interacting with LAIR-1 expressed on immune cells, and that LAIR-2 releases LAIR-1 mediated immune suppression.

Evaluation of public human datasets present that collagens, LAIR-1 and LAIR-2 have distinctive and overlapping associations with survival in sure tumors. We designed a dimeric LAIR-2 with a useful IgG1 Fc tail, NC410, and confirmed that NC410 will increase human T cell enlargement and effector operate in vivo in a mouse xenogeneic-graft versus-host illness mannequin. In humanized mouse tumor fashions NC410 reduces tumor development that’s depending on T cells. Immunohistochemical evaluation of human tumors exhibits that NC410 binds to collagen-rich areas the place LAIR-1+ immune cells are localized. Our findings present that NC410 could be a novel technique for most cancers immunotherapy for immune-excluded tumors.

Immunological Identification and Characterization of the Capsid Scaffold Protein Encoded by UL26.5 of Herpes Simplex Virus Sort 2

Herpes simplex virus sort 2 (HSV2), a pathogen that causes genital herpes lesions, interferes with the host immune system by way of numerous recognized and unknown mechanisms. This virus has been used to review viral antigenic composition. Convalescent serum from HSV2-infected sufferers was used to establish viral antigens by way of 2-D protein electrophoresis and immunoblotting. The serum predominantly acknowledged a number of capsid scaffold proteins encoded by gene UL26.5, primarily ICP35.

This protein has been primarily reported to operate quickly in viral meeting however isn’t expressed in mature virus particles. Additional immunological research advised that this protein elicits particular antibody and cytotoxic T lymphocyte (CTL) responses in mice, however these responses don’t end in a scientific protecting impact in response to HSV2 problem. The information advised that immunodominance of ICP35 could be used to design an built-in antigen with different viral glycoproteins.

 

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natx-ray

Autoimmunomic Signatures of Ageing and Age-Associated Neurodegenerative Ailments Are Related With Mind Perform and Ribosomal Proteins

Organic getting old is a fancy course of featured by declined operate of cells and tissues, together with these of the immune system. As a consequence, getting old impacts the expression and growth of autoantibodies and autoreactive T cells, which may be seen of their sum because the autoimmunome of a person. On this examine we analyzed whether or not units of autoimmune options are related to particular phenotypes which kind autoimmunomic signatures associated to age and neurodegenerative ailments. The autoantibody profile knowledge of wholesome topics and sufferers from the GEO database was used to discover autoimmunomic signatures of getting old and three neurodegenerative ailments together with Parkinson’s illness (PD), Alzheimer illness (AD) and A number of Sclerosis (MS).

Our outcomes reveal that the autoimmunomic signature of getting old is featured by an undulated enhance of IgG autoantibodies related to studying and conduct and a constant enhance of IgG autoantibodies associated to ribosome and translation, and the autoimmunomic signature of getting old are additionally related to age-related neurodegenerative ailments. Intriguingly, Differential Expression-Sliding Window Evaluation (DE-SWAN) recognized three waves of adjustments of autoantibodies throughout getting old at an age of 30, 50, and 62 years, respectively. Moreover, IgG autoantibodies, particularly these in opposition to ribosomal proteins, might be used as prediction markers for getting old and age-related neurodegenerative ailments. Subsequently, this examine for the primary time uncovers complete autoimmunomic signatures for getting old and age-related neurodegenerative ailments.

Identification of seven 000-9 000 Proteins from Cell Strains and Tissues by Single-Shot Microflow LC-MS/MS

A present pattern in proteomics is to accumulate knowledge in a “single-shot” by LC-MS/MS as a result of it simplifies workflows and guarantees higher throughput and quantitative accuracy than schemes that contain in depth pattern fractionation. Nonetheless, single-shot approaches can endure from restricted proteome protection when carried out by knowledge dependent acquisition (ssDDA) on nanoflow LC techniques. For purposes the place pattern portions aren’t scarce, this examine exhibits that top proteome protection may be obtained utilizing a microflow LC-MS/MS system working a 1 mm i.d. × 150 mm column, at a flow-rate of 50 μL/min and paired to an Orbitrap HF-X mass spectrometer.

The outcomes reveal the identification of ∼9 000 proteins from 50 μg of protein digest from Arabidopsis roots, 7 500 from mouse thymus, and seven 300 from human breast most cancers cells in three h of research time in a single run. The dynamic vary of protein quantification measured by the iBAQ strategy spanned 5 orders of magnitude and replicate evaluation confirmed that the median coefficient of variation was under 20%. Collectively, this examine exhibits that ssDDA by μLC-MS/MS is a strong technique for complete and large-scale proteome evaluation and which can be additional prolonged to extra speedy chromatography and knowledge unbiased acquisition approaches sooner or later.

 

Rat Heterogeneous nuclear ribonucleoproteins A2 ELISA kit

E02H0260-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Rat Heterogeneous nuclear ribonucleoproteins A2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Heterogeneous nuclear ribonucleoproteins A2 ELISA kit

E02H0260-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Rat Heterogeneous nuclear ribonucleoproteins A2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Heterogeneous nuclear ribonucleoproteins A2 ELISA kit

E03H0260-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Mouse Heterogeneous nuclear ribonucleoproteins A2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Heterogeneous nuclear ribonucleoproteins A2 ELISA kit

E03H0260-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Mouse Heterogeneous nuclear ribonucleoproteins A2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Heterogeneous nuclear ribonucleoproteins A2 ELISA kit

E03H0260-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Mouse Heterogeneous nuclear ribonucleoproteins A2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Heterogeneous nuclear ribonucleoproteins A2 ELISA kit

E04H0260-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rabbit Heterogeneous nuclear ribonucleoproteins A2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Heterogeneous nuclear ribonucleoproteins A2 ELISA kit

E04H0260-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rabbit Heterogeneous nuclear ribonucleoproteins A2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Heterogeneous nuclear ribonucleoproteins A2 ELISA kit

E04H0260-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rabbit Heterogeneous nuclear ribonucleoproteins A2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Heterogeneous nuclear ribonucleoproteins A2 ELISA kit

E06H0260-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Goat Heterogeneous nuclear ribonucleoproteins A2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Heterogeneous nuclear ribonucleoproteins A2 ELISA kit

E06H0260-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Goat Heterogeneous nuclear ribonucleoproteins A2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Heterogeneous nuclear ribonucleoproteins A2 ELISA kit

E06H0260-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Goat Heterogeneous nuclear ribonucleoproteins A2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Heterogeneous nuclear ribonucleoproteins A2 ELISA kit

E01H0260-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Human Heterogeneous nuclear ribonucleoproteins A2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Heterogeneous nuclear ribonucleoproteins A2 ELISA kit

E01H0260-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Human Heterogeneous nuclear ribonucleoproteins A2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Heterogeneous nuclear ribonucleoproteins A2 ELISA kit

E01H0260-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Human Heterogeneous nuclear ribonucleoproteins A2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Heterogeneous nuclear ribonucleoproteins A2 ELISA kit

E09H0260-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Monkey Heterogeneous nuclear ribonucleoproteins A2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Heterogeneous nuclear ribonucleoproteins A2 ELISA kit

E09H0260-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Monkey Heterogeneous nuclear ribonucleoproteins A2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Heterogeneous nuclear ribonucleoproteins A2 ELISA kit

E09H0260-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Monkey Heterogeneous nuclear ribonucleoproteins A2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Heterogeneous nuclear ribonucleoproteins A2 ELISA kit

E08H0260-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Canine Heterogeneous nuclear ribonucleoproteins A2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Heterogeneous nuclear ribonucleoproteins A2 ELISA kit

E08H0260-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Canine Heterogeneous nuclear ribonucleoproteins A2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Heterogeneous nuclear ribonucleoproteins A2 ELISA kit

E08H0260-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Canine Heterogeneous nuclear ribonucleoproteins A2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Heterogeneous nuclear ribonucleoproteins A2 ELISA kit

E07H0260-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Porcine Heterogeneous nuclear ribonucleoproteins A2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

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