Quickly rising SARS-CoV-2 B.1.1.7 sub-lineage in the USA of America with spike protein D178H and membrane protein V70L mutations
Summary The SARS-CoV-2 B.1.1.7 lineage is extremely infectious and as of April 2021 accounted for 92% of COVID-19 circumstances in Europe and 59% of COVID-19 circumstances within the U.S. It’s outlined by the N501Y mutation within the receptor binding area (RBD) of the Spike (S) protein, and some different mutations. These embrace two mutations within the N terminal area (NTD) of the S protein, HV69-70del and Y144del (often known as Y145del because of the presence of tyrosine at each positions). We lately recognized a number of rising SARS-CoV-2 variants of considerations, characterised by Membrane (M) protein mutations, together with I82T and V70L. We now determine a sub-lineage of B.1.1.7 that emerged by means of sequential acquisitions of M:V70L in November 2020 adopted by a novel S:D178H mutation first noticed in early February 2021.
The proportion of B.1.1.7 isolates within the U.S. that belong to this sub-lineage elevated from 0.15% in February 2021 to 1.8% in April 2021. So far this sub-lineage seems to be U.S.-specific with reported circumstances in 31 states, together with Hawaii. As of April 2021 it constituted 36.8% of all B.1.1.7 isolates in Washington. Phylogenetic evaluation and transmission inference with Nextstrain suggests this sub-lineage possible originated in both California or Washington. Structural evaluation revealed that the S:D178H mutation is within the NTD of the S protein and shut to 2 different signature mutations of B.1.1.7, HV69-70del and Y144del. It’s floor uncovered and will alter NTD tertiary configuration or accessibility, and thus has the potential to have an effect on neutralization by NTD directed antibodies.
Speedy three-dimensional form willpower of globular proteins by mobility capillary electrophoresis and native mass spectrometry
Established high-throughput proteomics strategies present restricted data on the stereostructures of proteins. Conventional applied sciences for protein construction willpower sometimes require laborious steps and can’t be carried out in a high-throughput style. Right here, we report a brand new medium throughput methodology by combining mobility capillary electrophoresis (MCE) and native mass spectrometry (MS) for the three-dimensional (3D) form willpower of globular proteins within the liquid section, which offers each the geometric construction and molecular mass data of proteins.
A principle was established to correlate the ion hydrodynamic radius and cost state distribution within the native mass spectrum with protein geometrical parameters, by means of which a low-resolution construction (form) of the protein might be decided. Our take a look at information of 11 completely different globular proteins confirmed that this method permits us to find out the shapes of particular person proteins, protein complexes and proteins in a mix, and to watch protein conformational modifications. Moreover offering complementary protein construction data and having combination evaluation functionality, this MCE and native MS primarily based methodology is quick in velocity and low in pattern consumption, making it probably relevant in top-down proteomics and structural biology for intact globular protein or protein advanced evaluation.
Quick floor immobilization of native proteins by means of catalyst-free amino-yne click on bioconjugation
Floor immobilization offers a helpful platform for biosensing, drug screening, tissue engineering and different chemical and organic functions. Nonetheless, a number of the used reactions are inefficient and/or sophisticated, limiting their functions in immobilization. Herein, we use a spontaneous and catalyst-free amino-yne click on bioconjugation to generate activated ethynyl group functionalized surfaces for quick immobilization of native proteins and cells.
Biomolecules, resembling bovine serum albumin (BSA), human IgG and a peptide of C(RGDfK), might be covalently immobilized on the surfaces in as brief as 30 min. Notably, the bioactivity of the anchored biomolecules stays intact, which is verified by effectively capturing goal antibodies and cells from the majority options. This technique represents an alternate for extremely environment friendly floor biofunctionalization.

On the binding response of loratadine with human serum acute section protein alpha 1-acid glycoprotein
Loratadine is a crucial anti-allergic drug. It’s a second era antihistamine drug used to deal with allergic rhinitis, hay fever and urticaria. Human serum alpha 1-acid glycoprotein (AG) is a crucial acute section protein and its serum focus is discovered to extend in irritation and acute response.The binding interplay between loratadine and AG is studied utilizing spectroscopy and molecular docking strategies. The outcomes obtained from fluorescence quenching experiments demonstrated that the fluorescence depth of AG is quenched by loratadine. Loratadine was discovered to bind AG with the binding fixed of ≈104 at 298 Ok.
The Gibb’s free power change was discovered to be destructive for the interplay of loratadine with AG indicating the binding course of is spontaneous. Binding of loratadine with AG induced ordered constructions within the protein. Hydrogen bonding and hydrophobic interactions had been the principle bonding forces between AG-loratadine as revealed by molecular docking outcomes. This research suggests the significance of binding of anti-allergic drug to AG spatially within the ailments the place the plasma focus of AG will increase many folds and interplay with this protein turns into important. This research will assist in design of drug dosage and adjustment accordingly to realize optimum therapy consequence. Communicated by Ramaswamy H. Sarma.
Focused Protein Degradation by means of Quick Optogenetic Activation and Its Utility to the Management of Cell Signaling
Improvement of methodologies for optically triggered protein degradation permits the research of dynamic protein capabilities, such as these concerned in cell signaling, which are troublesome to be probed with conventional genetic strategies. Right here, we describe the design and implementation of a novel light-controlled peptide degron conferring N-end pathway degradation to its protein goal. The degron includes a photocaged N-terminal amino acid and a lysine-rich, 13-residue linker. By caging the N-terminal residue, we had been in a position to optically management N-degron recognition by an E3 ligase, consequently controlling ubiquitination and proteasomal degradation of the goal protein.
We reveal broad applicability by making use of this method to a various set of goal proteins, together with EGFP, firefly luciferase, the kinase MEK1, and the phosphatase DUSP6 (often known as MKP3). The caged degron can be utilized with minimal protein engineering and offers nearly full, light-triggered protein degradation on a second to minute time scale.
Goat Milk Proteins Sample Diluent |
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F243A-100 | Cygnus Technologies | 100 ml | EUR 308.4 |
Description: Goat Milk Proteins Sample Diluent by Cygnus Technologies is available in Europe via Gentaur. |
Goat Milk Proteins Sample Diluent |
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F243A-500 | Cygnus Technologies | 500 ml | EUR 493.2 |
Description: Goat Milk Proteins Sample Diluent by Cygnus Technologies is available in Europe via Gentaur. |
Goat Milk Proteins Antigen Concentrate |
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F243H | Cygnus Technologies | 100 ul | EUR 493.2 |
Description: Goat Milk Proteins Antigen Concentrate by Cygnus Technologies is available in Europe via Gentaur. |
Goat Milk Proteins Control Antigen |
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F247 | Cygnus Technologies | 50 ul | EUR 308.4 |
Description: Goat Milk Proteins Control Antigen by Cygnus Technologies is available in Europe via Gentaur. |
Total Goat Milk Proteins ELISA kit |
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F240 | Cygnus Technologies | 1 kit (96 wells plate) | EUR 1296 |
Description: Total Goat Milk Proteins ELISA kit by Cygnus Technologies is available in Europe via Gentaur. |
Total Goat Milk Proteins WB kit |
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F245 | Cygnus Technologies | 1 kit (application dependant amount of reactions) | EUR 1003.2 |
Description: Total Goat Milk Proteins WB kit by Cygnus Technologies is available in Europe via Gentaur. |
Rabbit Polyclonal antibody Anti-CRBN |
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Anti-CRBN | ImmunoStep | 50 µg | EUR 418.8 |
Human milk specific allergen antibody(IgE) ELISA Kit |
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CSB-E15859h-24T | Cusabio | 1 plate of 24 wells | EUR 198 |
Description: Quantitativesandwich ELISA kit for measuring Human milk specific allergen antibody (IgE) in samples from serum, plasma, cell culture supernates, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price. |
Human milk specific allergen antibody(IgE) ELISA Kit |
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1-CSB-E15859h | Cusabio |
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Description: Quantitativesandwich ELISA kit for measuring Human milk specific allergen antibody(IgE) in samples from serum, plasma, cell culture supernates, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits. |
Anti-milk fat globulin antibody |
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STJ16100177 | St John's Laboratory | 100 µg | EUR 487.2 |
Anti-Goat Milk Proteins:HRP Conjugate |
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F241 | Cygnus Technologies | 12 ml | EUR 585.6 |
Description: Anti-Goat Milk Proteins:HRP Conjugate by Cygnus Technologies is available in Europe via Gentaur. |
Anti-Bovine Plasma Proteins |
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BPP04 | Cygnus Technologies | 1 ml | EUR 400.8 |
Description: Anti-Bovine Plasma Proteins by Cygnus Technologies is available in Europe via Gentaur. |
Anti-Bovine Plasma Proteins |
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BPP04-AF | Cygnus Technologies | 1 mg | EUR 2295.6 |
Description: Anti-Bovine Plasma Proteins by Cygnus Technologies is available in Europe via Gentaur. |
Anti-Bovine Plasma Proteins |
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BPP04-PA | Cygnus Technologies | 1 ml | EUR 585.6 |
Description: Anti-Bovine Plasma Proteins by Cygnus Technologies is available in Europe via Gentaur. |
Anti-Bovine Plasma Proteins |
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BPP04AF-HRP | Cygnus Technologies | 100 ul | EUR 1186.8 |
Description: Anti-Bovine Plasma Proteins by Cygnus Technologies is available in Europe via Gentaur. |
Skim Milk |
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abx082021-100g | Abbexa | 100 g | EUR 260.4 |
Polyclonal Goat anti-GST α-form |
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GST-ANTI-1 | Detroit R&D | 50 uL | EUR 336 |
Polyclonal Goat anti-GST μ-form |
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GST-ANTI-2 | Detroit R&D | 50 uL | EUR 336 |
Polyclonal Goat anti-GST p-form |
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GST-ANTI-3 | Detroit R&D | 50 uL | EUR 336 |
Lys proteins Antibody |
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DF2705 | Affbiotech | 200ul | EUR 420 |
Lys proteins Antibody |
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ABD2705 | Lifescience Market | 100 ug | EUR 525.6 |
Lys proteins Antibody |
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abx219526-100ug | Abbexa | 100 ug | EUR 526.8 |
anti-Human Milk Fat Globule 1 |
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YF-PA24148 | Abfrontier | 50 ul | EUR 400.8 |
Description: Mouse polyclonal to Human Milk Fat Globule 1 |
anti-Human Milk Fat Globule 1 |
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YF-PA13135 | Abfrontier | 50 ug | EUR 435.6 |
Description: Mouse polyclonal to Human Milk Fat Globule 1 |
anti-Human Milk Fat Globule 1 |
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YF-PA13136 | Abfrontier | 100 ul | EUR 483.6 |
Description: Rabbit polyclonal to Human Milk Fat Globule 1 |
anti-Human Milk Fat Globule 1 |
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YF-PA13137 | Abfrontier | 100 ug | EUR 483.6 |
Description: Rabbit polyclonal to Human Milk Fat Globule 1 |
Anti-Human Milk Fat Globule 1/MFGE8 Antibody |
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PA1945 | BosterBio | 100ug/vial | EUR 400.8 |
anti- Lin28A-specific antibody |
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FNab04784 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against Lin28A-specific |
anti- Lin28B-specific antibody |
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FNab04786 | FN Test | 100µg | EUR 702 |
Description: Antibody raised against Lin28B-specific |
anti- LRP2-Specific antibody |
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FNab04847 | FN Test | 100µg | EUR 702 |
Description: Antibody raised against LRP2-Specific |
anti- MAS1L-Specific antibody |
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FNab05020 | FN Test | 100µg | EUR 702 |
Description: Antibody raised against MAS1L-Specific |
anti- MCL1L-specific antibody |
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FNab05054 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against MCL1L-specific |
anti- MDM1-Specific antibody |
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FNab05076 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against MDM1-Specific |
anti- MEF2C-Specific antibody |
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FNab05107 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against MEF2C-Specific |
anti- MYH10-Specific antibody |
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FNab05472 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against MYH10-Specific |
anti- MYH4-Specific antibody |
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FNab05476 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against MYH4-Specific |
anti- MYH7-specific antibody |
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FNab05478 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against MYH7-specific |
anti- MYOM1-Specific antibody |
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FNab05516 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against MYOM1-Specific |
anti- MYPN-Specific antibody |
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FNab05525 | FN Test | 100µg | EUR 702 |
Description: Antibody raised against MYPN-Specific |
anti- MYSM1-Specific antibody |
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FNab05527 | FN Test | 100µg | EUR 702 |
Description: Antibody raised against MYSM1-Specific |
anti- NARFL-Specific antibody |
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FNab05553 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against NARFL-Specific |
anti- NEB-Specific antibody |
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FNab05638 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against NEB-Specific |
anti- Neogenin-Specific antibody |
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FNab05662 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against Neogenin-Specific |
anti- neurturin-Specific antibody |
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FNab05683 | FN Test | 100µg | EUR 702 |
Description: Antibody raised against neurturin-Specific |
anti- NFIX-Specific antibody |
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FNab05701 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against NFIX-Specific |
anti- NOX3-Specific antibody |
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FNab05805 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against NOX3-Specific |
anti- OCT2-Specific antibody |
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FNab05962 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against OCT2-Specific |
anti- Oct6-Specific antibody |
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FNab05965 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against Oct6-Specific |
anti- ORM2-Specific antibody |
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FNab06014 | FN Test | 100µg | EUR 702 |
Description: Antibody raised against ORM2-Specific |
anti- PACS2-Specific antibody |
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FNab06099 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against PACS2-Specific |
anti- Palladin-Specific antibody |
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FNab06127 | FN Test | 100µg | EUR 702 |
Description: Antibody raised against Palladin-Specific |
anti- PAX1-Specific antibody |
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FNab06167 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against PAX1-Specific |
anti- PAX8-Specific antibody |
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FNab06177 | FN Test | 100µg | EUR 702 |
Description: Antibody raised against PAX8-Specific |
anti- persephin-Specific antibody |
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FNab06323 | FN Test | 100µg | EUR 702 |
Description: Antibody raised against persephin-Specific |
anti- PFKFB3-Specific antibody |
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FNab06339 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against PFKFB3-Specific |
anti- PKM1-specific antibody |
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FNab06494 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against PKM1-specific |
anti- PKM2-specific antibody |
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FNab06495 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against PKM2-specific |
anti- PKM2-specific antibody |
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FNab06496 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against PKM2-specific |
anti- PLOD2-Specific antibody |
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FNab06552 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against PLOD2-Specific |
anti- POLR2B-Specific antibody |
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FNab06620 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against POLR2B-Specific |
anti- POTEA-Specific antibody |
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FNab06647 | FN Test | 100µg | EUR 702 |
Description: Antibody raised against POTEA-Specific |
anti- POTED-Specific antibody |
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FNab06648 | FN Test | 100µg | EUR 702 |
Description: Antibody raised against POTED-Specific |
anti- POU3F3-Specific antibody |
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FNab06651 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against POU3F3-Specific |
anti- PPIF-Specific antibody |
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FNab06676 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against PPIF-Specific |
anti- PPP1CB-Specific antibody |
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FNab06695 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against PPP1CB-Specific |
anti- PPP1R11-Specific antibody |
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FNab06699 | FN Test | 100µg | EUR 702 |
Description: Antibody raised against PPP1R11-Specific |
anti- PSEN2-Specific antibody |
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FNab06853 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against PSEN2-Specific |
anti- PTH-Specific antibody |
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FNab06923 | FN Test | 100µg | EUR 702 |
Description: Antibody raised against PTH-Specific |
anti- PYGM-Specific antibody |
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FNab06977 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against PYGM-Specific |
anti- RAB11B-Specific antibody |
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FNab06989 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against RAB11B-Specific |
anti- RAB11-Specific antibody |
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FNab06994 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against RAB11-Specific |
anti- RAB22A-specific antibody |
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FNab07005 | FN Test | 100µg | EUR 702 |
Description: Antibody raised against RAB22A-specific |
anti- RAB27A-specific antibody |
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FNab07011 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against RAB27A-specific |
anti- RAB31-specific antibody |
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FNab07015 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against RAB31-specific |
anti- RAB3A-specific antibody |
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FNab07025 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against RAB3A-specific |
anti- RAB3C-specific antibody |
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FNab07028 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against RAB3C-specific |
anti- RAB5A-Specific antibody |
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FNab07039 | FN Test | 100µg | EUR 702 |
Description: Antibody raised against RAB5A-Specific |
anti- RAB9A-Specific antibody |
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FNab07049 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against RAB9A-Specific |
anti- REDD1 specific antibody |
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FNab07228 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against REDD1 specific |
anti- RIM2-Specific antibody |
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FNab07301 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against RIM2-Specific |
anti- RIMS4-Specific antibody |
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FNab07305 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against RIMS4-Specific |
anti- RIPK1-Specific antibody |
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FNab07313 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against RIPK1-Specific |
anti- ROBO1-Specific antibody |
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FNab07372 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against ROBO1-Specific |
anti- ROBO3-Specific antibody |
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FNab07374 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against ROBO3-Specific |
anti- ROBO4-Specific antibody |
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FNab07375 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against ROBO4-Specific |
anti- ROCK1-Specific antibody |
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FNab07377 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against ROCK1-Specific |
anti- ROCK2-Specific antibody |
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FNab07379 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against ROCK2-Specific |
anti- RRM1-Specific antibody |
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FNab07495 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against RRM1-Specific |
anti- RtcB-Specific antibody |
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FNab07515 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against RtcB-Specific |
anti- SEC61B-Specific antibody |
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FNab07691 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against SEC61B-Specific |
anti- SENP5-Specific antibody |
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FNab07714 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against SENP5-Specific |
anti- SERINC2-Specific antibody |
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FNab07736 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against SERINC2-Specific |
anti- ATP5G2-Specific antibody |
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FNab00708 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against ATP5G2-Specific |
anti- BAG1S-specific antibody |
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FNab00788 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against BAG1S-specific |
anti- BAI2-Specific antibody |
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FNab00791 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against BAI2-Specific |
anti- BAI3-Specific antibody |
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FNab00792 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against BAI3-Specific |
anti- Barkor-Specific antibody |
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FNab00806 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against Barkor-Specific |
anti- BLOC1S1-Specific antibody |
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FNab00909 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against BLOC1S1-Specific |
anti- BRN2-Specific antibody |
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FNab00963 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against BRN2-Specific |
anti- CAPG-Specific antibody |
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FNab01249 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against CAPG-Specific |
anti- Catalase-Specific antibody |
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FNab01302 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against Catalase-Specific |
anti- CCKAR-specific antibody |
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FNab01377 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against CCKAR-specific |
anti- CCKBR-specific antibody |
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FNab01378 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against CCKBR-specific |
anti- CCR2-specific antibody |
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FNab01390 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against CCR2-specific |
anti- CCT6A-Specific antibody |
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FNab01401 | FN Test | 100µg | EUR 606.3 |
Description: Antibody raised against CCT6A-Specific |