The coronavirus illness 2019 (COVID-19) brought on by a novel coronavirus, SARS-CoV-2, has contaminated greater than 50.6 million people and prompted over 1.2 million deaths globally, elevating a serious well being concern. Up to now, no particular antiviral therapy or vaccine for COVID-19 has been accredited by the Meals and Drug Administration (FDA). Extremely delicate and particular laboratory diagnostics are due to this fact vital for controlling the quickly evolving COVID-19 pandemic and optimizing scientific care, an infection management, and public well being interventions.
The FDA has issued emergency use authorization (EUA) for tons of of COVID-19 diagnostic checks of various courses. Whereas nucleic acid testing (NAT) comparable to RT–PCR stays the criterion customary for COVID-19 prognosis, serological antibody and antigen checks are more and more being developed. Assessments primarily based on the novel RNA sensing strategies (e.g., SHERLOCK, DETECTR, and Toehold Change) are promising because of their comparatively low value, excessive accuracy, and fast detection time.
Diagnostic testing outcomes for SARS-CoV-2 must be interpreted with warning, since they rely closely on elements comparable to viral load, virus replication, the supply and timing of pattern assortment, pattern extraction, and traits of varied testing strategies. This overview goals to current the present standing of frequent diagnostic testing for SARS-CoV-2 an infection, overview the present regulatory necessities, and determine future instructions within the improvement of improved diagnostics which might be extra correct, accessible, and fast.
The position and efficiency of chest CT within the prognosis of the coronavirus illness 2019 pandemic stays beneath lively investigation. A current case report on this journal discovered {that a} COVID-19 prognosis has been constructed from the serological outcomes regardless of first CT scan and RT–PCR damaging outcomes. We now have included 311 consecutive sufferers with COVID-19-like signs who underwent a RT–PCR take a look at on nasopharyngeal swab samples and a chest CT scan.
Sufferers with a constructive chest scan and a damaging RT–PCR have been screened for serum whole anti-SARS-CoV-2 antibodies 2 months after the onset of signs utilizing the Wantai take a look at in an effort to arbitrate between RT–PCR and CT scans discordant outcomes. A lot of the RT–PCR and chest CT outcomes agreed however the serological checks point out that the majority CT constructive/NAT damaging sufferers should not contaminated by SARS-CoV-2 and various diagnoses must be significantly thought-about. This text is protected by copyright. All rights reserved.
CT Quantification and Machine-learning Fashions for Evaluation of Illness Severity and Prognosis of COVID-19 Sufferers
Ninety-nine COVID-19 sufferers who have been confirmed by constructive nucleic acid take a look at (NAT) of RT–PCR and hospitalized from January 19, 2020 to February 19, 2020 have been collected for this retrospective research. All sufferers underwent arterial blood gasoline take a look at, routine blood take a look at, chest CT examination, and bodily examination on admission. As well as, follow-up scientific knowledge together with the illness severity, scientific therapy, and scientific outcomes have been collected for every affected person.
Lung quantity, lesion quantity, nonlesion lung quantity (NLLV) (lung quantity – lesion quantity), and fraction of nonlesion lung quantity (%NLLV) (nonlesion lung quantity / lung quantity) have been quantified in CT photographs by utilizing two U-Internet fashions educated for segmentation of lung and COVID-19 lesions in CT photographs. Moreover, we calculated 20 histogram textures for lesions quantity and NLLV, respectively.
To research the validity of CT quantification within the administration of COVID-19, we constructed random forest (RF) fashions for the aim of classification and regression to evaluate the illness severity (Average, Extreme, and Essential) and to foretell the necessity and size of ICU keep, the period of oxygen inhalation, hospitalization, sputum NAT-positive, and affected person prognosis. The efficiency of RF classifiers was evaluated utilizing the realm beneath the receiver working attribute curves (AUC) and that of RF regressors utilizing the root-mean-square error.
Expression and Clinicopathological Significances of lncRNAs: Might ARA and ZEB2NAT be the Potential Breast Most cancers-Associated Biomarkers?
Items of proof have proven {that a} important proportion of cancer-prone elements should not attributed to alterations in protein-coding sequences. Adriamycin resistance-related (ARA) and pure antisense of ZEB2 (ZEB2NAT) lengthy non-coding RNAs (lncRNAs) have been indicated with oncogenic properties by regulating numerous signaling pathways and epithelial-to-mesenchymal transition (EMT), which can have diagnostic and prognostic potential as a novel group of biomarkers. The present research aimed to judge the expression standing of ARA and ZEB2NAT lncRNAs and their clinicopathological significance in a inhabitants with breast most cancers (BC).
Our knowledge confirmed a big upregulation of ARA and ZEB2NAT lncRNAs in tumor tissues in comparison with NATs (p <0.001; p = 0.021, respectively). ARA and ZEB2NAT expression have been noticed to be considerably related to tumor grade, nuclear grade, tumor phases, and lymph node metastasis (p <0.05). Moreover, ARA expression was considerably correlated with breastfeeding standing (p = 0.027). our knowledge revealed that ARA and ZEB2NAT lncRNAs have been overexpressed in BC. Moreover, the chosen lncRNAs have been discovered to could be the potential biomarkers for BC prognosis and prognosis. Nonetheless, the findings of the present analysis are required to be replicated in different research with bigger pattern sizes.
Measles Virus PCR kit |
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| PCR-H639-96R | Bioingentech | 100T | EUR 1335.6 |
Measles Virus One-Step PCR kit |
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Measles Virus One-Step PCR kit |
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Measles Virus One-Step PCR kit |
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Rubella Virus Real Time RT-PCR Kit |
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Rubella Virus Real Time RT-PCR Kit |
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Chikungunya Virus Real Time RT-PCR Kit |
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Chikungunya Virus Real Time RT-PCR Kit |
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| GWB-LRB002 | GenWay Biotech | 25 tests | Ask for price |
Monkeypox Virus Real Time PCR Kit |
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| YJC70115NW-25T | Jiangsu Bioperfectus Technologies | 25 tests/kit | Ask for price |
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Description: The Bioperfectus Monkeypox Virus Real Time PCR Kit is an in vitro diagnostic test, based on real-time PCR technology, for the detection of DNA from the Monkeypox virus. Specimens can be obtained from human serum, lesion exudate samples and scab. BSL-2 facilities with standard BSL-2 work practices may be used for the test of t he Monkeypox virus. |
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Monkeypox Virus Real Time PCR Kit |
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| ZD-0076-01 | Gentaur Genprice | 25 tests/kit | Ask for price |
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Description: Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids. The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long. The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of the Monkeypox Virus DNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channel 530nm with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the 560nm fluorescence of the internal control (IC). An external positive control defined as 1×10^7 copies/ml is supplied which allow the determination of the gene load. |
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Monkeypox Virus Real Time PCR Kit |
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| ZD-0076-02 | Gentaur Genprice | 25 tests/kit | Ask for price |
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Description: Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids.The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long.The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of theMonkeypox VirusDNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channelFAM with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the HEX/VIC/JOE fluorescence of the internal control (IC). An external positive control defined as 1×107copies/ml is supplied which allow the determination of the gene load. |
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Monkeypox Virus Real Time PCR Kit |
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| PDPS-AR064 | Creative Biogene | 1 unit | Ask for price |
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Description: Creative Biogene Monkeypox Virus Real Time PCR Kit is used for the detection of monkeypox Virus in serum or lesion exudate samples by using real time PCR systems. Monkeypox virus (MPV) is a double-stranded DNA, zoonotic virus and a species of the genus Orthopoxvirus in the family Poxviridae. It is one of the human orthopoxviruses that includes variola (VARV), cowpox (CPX), and vaccinia (VACV) viruses. The kit contains a specific ready-to-use system for the detection of the monkeypox Virus. Fluorescence is emitted and measured by the real time systems' optical unit during the PCR. |
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Canine Distemper virus Real Time RT-PCR Detection kit |
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| TRI-L10M1 | TRI Biotech | 32T | EUR 705.6 |
Canine Distemper virus Real Time RT-PCR Detection kit |
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Yellow head Virus Real Time PCR Detection Kit |
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Yellow head Virus Real Time PCR Detection Kit |
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Taura Syndrome Virus Real Time PCR Detection Kit |
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Taura Syndrome Virus Real Time PCR Detection Kit |
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Feline leukemia virus Real Time PCR Detection kit |
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Feline leukemia virus Real Time PCR Detection kit |
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Transfusion-transmitted Virus (TTV) Real Time PCR Kit |
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| GWB-LRB041 | GenWay Biotech | 25 tests | Ask for price |
Transfusion-transmitted Virus (TTV) Real Time PCR Kit |
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ViPrimePLUS Human Measles Virus RT-qPCR Kit, 150rxns |
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| QM5034 | Vivantis | each | Ask for price |
Measles Virus |
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| MBS319569-1mg | MyBiosource | 1mg | EUR 930 |
Measles Virus |
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Measles Virus |
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| MBS319615-1mg | MyBiosource | 1mg | EUR 930 |
Measles Virus |
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Measles Virus |
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Measles Virus |
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| MBS319630-5x1mg | MyBiosource | 5x1mg | EUR 4020 |
Measles Virus |
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Measles Virus |
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Viral Hemorrhagic Septicemia Virus Real Time RT-PCR Detection kit |
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| TRI-N16M1 | TRI Biotech | 32T | EUR 577.08 |
Viral Hemorrhagic Septicemia Virus Real Time RT-PCR Detection kit |
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| TRI-N16S1 | TRI Biotech | 16T | EUR 327.6 |
White Spot Syndrome Virus Real Time PCR Detection Kit |
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| TRI-N01M1 | TRI Biotech | 32T | EUR 453.6 |
White Spot Syndrome Virus Real Time PCR Detection Kit |
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Dengue Real-TM Real Time PCR kit for detection of Dengue Virus |
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Macrobrachium rosenbergii Norda Virus Real Time RT-PCR Detection kit |
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| TRI-N13M1 | TRI Biotech | 32T | EUR 577.08 |
Macrobrachium rosenbergii Norda Virus Real Time RT-PCR Detection kit |
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Zika Virus Real-TM Real Time PCR test for detection of Zika Virus |
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| V73-50FRT | Sacace Biotechnologies | 50 | EUR 1257.86 |
Shrimp Hemocyte Iridescent Virus Real Time PCR Detection Kit |
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| TRI-N03M1 | TRI Biotech | 32T | EUR 453.6 |
Shrimp Hemocyte Iridescent Virus Real Time PCR Detection Kit |
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| TRI-N03S1 | TRI Biotech | 16T | EUR 252 |
Measles Virus IgG |
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| DEMEAG0330 | Demeditec Diagnostics | 96 | EUR 92 |
Measles Virus IgM |
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| DEMEAM0330 | Demeditec Diagnostics | 96 | EUR 92 |
Measles Virus IgG |
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Measles Virus IgM |
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Measles Virus IgM |
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| MBS191656-3mL | MyBiosource | 3mL | EUR 230 |
Measles Virus IgM |
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| MBS191656-5x3mL | MyBiosource | 5x3mL | EUR 980 |
Spring Viraemia of Carp Virus Real Time PCR Detection kit |
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| TRI-N17M1 | TRI Biotech | 32T | EUR 577.08 |
Spring Viraemia of Carp Virus Real Time PCR Detection kit |
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| TRI-N17S1 | TRI Biotech | 16T | EUR 327.6 |
Measles Virus IgG ELISA Kit |
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| ESR102G | Genovis AB | 96 Tests | EUR 372 |
Measles Virus IgM ELISA Kit |
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| ESR102M | Genovis AB | 96 Tests | EUR 372 |
Measles Virus IgG ELISA Kit |
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| MBS495631-5x96Tests | MyBiosource | 5x96Tests | EUR 1665 |
Measles Virus IgG ELISA Kit |
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| MBS495631-96Tests | MyBiosource | 96Tests | EUR 365 |
NATIVE MEASLES VIRUS |
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| GWB-3EA72C | GenWay Biotech | 1 ml | Ask for price |
Measles virus lysate |
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| NAT41576-100 | The Native Antigen Company | 0.1 | EUR 512.85 |
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Description: Purified Measles virus lysate. |
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NATIVE MEASLES VIRUS |
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| MBS239121-1mL | MyBiosource | 1mL | EUR 390 |
NATIVE MEASLES VIRUS |
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| MBS239121-5x1mL | MyBiosource | 5x1mL | EUR 1520 |
Measles virus protein |
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| 30-1308 | Fitzgerald | 1 mg | EUR 200 |
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Description: Purified native Measles virus protein |
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Measles Virus Antigen |
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| PR-BA102-L | Jena Bioscience GmbH | 1mg | EUR 961.8 |
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Description: native, Vero cell culture |
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Measles Virus Antigen |
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| PR-BA102-S | Jena Bioscience GmbH | 250µg | EUR 321 |
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Description: native, Vero cell culture |
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Measles Virus Antigen |
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| BA102VS | Genovis AB | 1mg | EUR 771 |
Measles Virus Antigen |
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| MBS568782-1mL | MyBiosource | 1mL | EUR 405 |
Measles Virus Antigen |
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| MBS568782-5x1mL | MyBiosource | 5x1mL | EUR 1545 |
Measles Virus Antigen |
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| MBS569315-1mL | MyBiosource | 1mL | EUR 620 |
Measles Virus Antigen |
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| MBS569315-5x1mL | MyBiosource | 5x1mL | EUR 2515 |
Measles virus protein |
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| MBS5303634-1mg | MyBiosource | 1mg | EUR 425 |
Measles virus protein |
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| MBS5303634-5x1mg | MyBiosource | 5x1mg | EUR 1680 |
Human Measles Virus ELISA Kit |
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| MBS3803382-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 6725 |
Human Measles Virus ELISA Kit |
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| MBS3803382-48StripWells | MyBiosource | 48-Strip-Wells | EUR 550 |
Human Measles Virus ELISA Kit |
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| MBS3803382-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 3420 |
Human Measles Virus ELISA Kit |
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| MBS3803382-96StripWells | MyBiosource | 96-Strip-Wells | EUR 765 |
Virus Measles Virus (MeV) Protein |
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| abx670263-1ml | Abbexa | 1 ml | EUR 543.6 |
Measles Virus Antibody |
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| abx023654-1mg | Abbexa | 1 mg | EUR 1053.6 |
Measles Virus Antibody |
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| abx023655-1mg | Abbexa | 1 mg | EUR 1053.6 |
Measles Virus Antibody |
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| abx023656-1mg | Abbexa | 1 mg | EUR 1053.6 |
Measles Virus Antibody |
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| abx023657-1mg | Abbexa | 1 mg | EUR 1053.6 |
Measles Virus Antibody |
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| abx023654-20l | Abbexa | 20 µl | EUR 1175 |
Measles Virus Antibody |
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| abx023654-400l | Abbexa | 400 µl | Ask for price |
Measles Virus Antibody |
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| abx023654-80l | Abbexa | 80 µl | Ask for price |
Measles Virus Antibody |
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| abx023655-400l | Abbexa | 400 µl | Ask for price |
Measles Virus Antibody |
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| abx023655-80l | Abbexa | 80 µl | EUR 1175 |
Measles Virus Antibody |
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| abx023656-400l | Abbexa | 400 µl | Ask for price |
Measles Virus Antibody |
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| abx023656-80l | Abbexa | 80 µl | EUR 1175 |
Measles Virus Antibody |
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| abx023657-400l | Abbexa | 400 µl | Ask for price |
Measles Virus Antibody |
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| abx023657-80l | Abbexa | 80 µl | EUR 1175 |
Measles Virus Antibody |
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| MBS568964-1mg | MyBiosource | 1mg | EUR 515 |
Measles Virus Antibody |
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| MBS568964-5x1mg | MyBiosource | 5x1mg | EUR 2110 |
Measles Virus Antibody |
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| MBS568965-1mg | MyBiosource | 1mg | EUR 515 |
Measles Virus Antibody |
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| MBS568965-5x1mg | MyBiosource | 5x1mg | EUR 2110 |
Measles Virus Nucleoprotein |
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| GWB-39AAEA | GenWay Biotech | 0.1 mg | Ask for price |
Measles Virus IgM ELISA |
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| MBS495656-5x96Tests | MyBiosource | 5x96Tests | EUR 1665 |
Measles Virus IgM ELISA |
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| MBS495656-96Tests | MyBiosource | 96Tests | EUR 365 |
Monkey measles virus,MV ELISA Kit |
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| YX-SL0040Mk | Sunlong | - | Ask for price |
Human Measles Virus IgG ELISA Kit |
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| abx055421-100g | Abbexa | 100 µg | Ask for price |
Human Measles Virus IgG ELISA Kit |
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| abx055421-10g | Abbexa | 10 µg | EUR 493.75 |
Human Measles Virus IgG ELISA Kit |
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| abx055421-50g | Abbexa | 50 µg | Ask for price |
Human Measles Virus IgM ELISA Kit |
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| abx052551-100g | Abbexa | 100 µg | Ask for price |
Human Measles Virus IgM ELISA Kit |
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| abx052551-10g | Abbexa | 10 µg | EUR 493.75 |
Human Measles Virus IgM ELISA Kit |
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| abx052551-50g | Abbexa | 50 µg | Ask for price |
Human Measles Virus IgM ELISA Kit |
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| MBS3803420-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 6725 |
Human Measles Virus IgM ELISA Kit |
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| MBS3803420-48StripWells | MyBiosource | 48-Strip-Wells | EUR 550 |
Human Measles Virus IgM ELISA Kit |
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| MBS3803420-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 3420 |
Human Measles Virus IgM ELISA Kit |
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| MBS3803420-96StripWells | MyBiosource | 96-Strip-Wells | EUR 765 |
Measles Virus (MeV) Protein |
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| abx670263-100g | Abbexa | 100 µg | EUR 362.5 |
West Nile Virus Real-TM Real Time PCR test for detection of WNV |
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| V53-50FRT | Sacace Biotechnologies | 50 | EUR 592.96 |
Real Time PCR kit |
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| B42-4-100FRT | Sacace Biotechnologies | 100 | EUR 981 |
Measles (Rubeola) virus |
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| MBS320370-01mg | MyBiosource | 0.1mg | EUR 355 |
Measles (Rubeola) virus |
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| MBS320370-5x01mg | MyBiosource | 5x0.1mg | EUR 1500 |
Measles (Rubeola) virus |
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| MBS320371-01mg | MyBiosource | 0.1mg | EUR 355 |
Measles (Rubeola) virus |
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| MBS320371-5x01mg | MyBiosource | 5x0.1mg | EUR 1500 |
Measles (Rubeola) virus |
|||
| MBS320372-01mg | MyBiosource | 0.1mg | EUR 355 |
Measles (Rubeola) virus |
|||
| MBS320372-5x01mg | MyBiosource | 5x0.1mg | EUR 1500 |
Measles (Rubeola) virus |
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| MBS320373-01mg | MyBiosource | 0.1mg | EUR 355 |
Measles (Rubeola) virus |
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| MBS320373-5x01mg | MyBiosource | 5x0.1mg | EUR 1500 |
Mouse anti Measles virus (6012) |
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| MAB12340-100 | The Native Antigen Company | 0.1 | EUR 275.56 |
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Description: Mouse monoclonal antibody specific for Measles Virus (clone 6012) |
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Mouse anti Measles virus (6012) |
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| MAB12340-500 | The Native Antigen Company | 0.5 | EUR 691.46 |
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Description: Mouse monoclonal antibody specific for Measles Virus (clone 6012) |
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Mouse anti Measles virus (6014) |
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| MAB12341-100 | The Native Antigen Company | 0.1 | EUR 275.56 |
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Description: Mouse monoclonal antibody specific for Measles Virus (clone 6014) |
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Mouse anti Measles virus (6014) |
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| MAB12341-500 | The Native Antigen Company | 0.5 | EUR 691.46 |
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Description: Mouse monoclonal antibody specific for Measles Virus (clone 6014) |
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Mouse anti Measles virus (6015) |
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| MAB12342-100 | The Native Antigen Company | 0.1 | EUR 275.56 |
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Description: Mouse monoclonal antibody specific for Measles Virus (clone 6015) |
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Mouse anti Measles virus (6015) |
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| MAB12342-500 | The Native Antigen Company | 0.5 | EUR 691.46 |
|
Description: Mouse monoclonal antibody specific for Measles Virus (clone 6015) |
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Mouse anti Measles virus (6017) |
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| MAB12343-100 | The Native Antigen Company | 0.1 | EUR 344.45 |
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Description: Mouse monoclonal antibody specific for Measles Virus (clone 6017) |
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Mouse anti Measles virus (6017) |
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| MAB12343-500 | The Native Antigen Company | 0.5 | EUR 691.46 |
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Description: Mouse monoclonal antibody specific for Measles Virus (clone 6017) |
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Research have demonstrated the diagnostic effectivity of antibody testing in COVID-19 an infection. There may be restricted knowledge on the IgM/IgG modifications in asymptomatic and discharged sufferers with reoccurring constructive nucleic acid take a look at (RPNAT). This research goals to research these IgM/IgG modifications.There have been 111 sufferers with constructive nucleic acid take a look at (NAT) and 40 suspected sufferers enrolled within the research. The serum SARS-CoV-2 particular IgM/IgG antibody ranges have been retrospectively analyzed with the illness progress in asymptomatic and RPNAT sufferers.