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Lanthanide derivatives are obtained with these compounds, either by soaking or by
co-crystallization, into crystals of biological macromolecules.
The crystal derivatives thus obtained can be used to solve the
structure of biological macromolecules by using structure-determination
methods taking advantage of the strong anomalous signal of lanthanides
in their LIII absorption edge or, for some of them, with radiation
from a lab X-ray source. Derivative based on Eu atom (LIII abs. edge: 6977 eV)
or Yb atom (LIII abs. edge: 8944 eV) are available.
We propose these compounds individually in larger quantity than in the phasing kit,
for the refinement of a promising derivative.
|
reference
|
compound
|
amount (mL)
|
concentration (mM)
|
|
CSM002-0101A
|
Eu-(DPA)3
|
1.0
|
200
|
|
CSM002-0102A
|
Yb-(DPA)3
|
1.0
|
200
|
|
CSM002-0103A
|
Eu-DOTA
|
0.2
|
500
|
|
CSM002-0104A
|
Yb-DOTA
|
0.2
|
500
|
|
CSM002-0105A
|
Eu-DO3A
|
0.2
|
500
|
|
CSM002-0106A
|
Yb-DO3A
|
0.2
|
500
|
|
CSM002-0107A
|
Eu-HPDO3A
|
0.2
|
500
|
|
CSM002-0108A
|
Yb-HPDO3A
|
0.2
|
500
|
|
CSM002-0109A
|
Eu-DTPA-BMA
|
0.2
|
500
|
|
CSM002-0110A
|
Yb-DTPA-BMA
|
0.2
|
500
|
Full documentation is available here.
The structure of these compounds is available on the
phasing kit page.
A list of publications related to the use of these complexes is available
here.
Authors publishing results thanks to these compounds are welcome
to cite the following papers:
For tris(dipicolinate)-lanthanide complexes:
Pompidor G, D'Aleo A, Vicat J, Toupet L, Giraud N, Kahn R, Maury O,
Angew Chem Int Ed Engl. 47 (2008), 3388-3391
For the other complexes:
Girard E, Stelter M, Vicat J, Kahn R,
Acta Cryst. D59 (2003), 1914-1922
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